Coding

Part:BBa_K3738003

Designed by: Emily Hagens and Mark Lea   Group: iGEM21_Lethbridge   (2021-10-17)


MS2 Bacteriophage Maturation Protein A with N-Terminal Histidine Tag and TEV Cleavage Site

Maturation Protein A is required in order for the capsid of the MS2 phage, and subsequently MS2-derived phage-like particles (PLP), to bind to bacterial pili. It is released from the capsid upon binding, and enters the cell along with the cargo to be delivered by the MS2 capsid. This maturation protein A coding region includes a histidine tag on the N-terminus, to allow for purification as a monomer for capsid assembly in vitro. There is also a TEV cleavage site between the coding region and the histidine tag, in order to allow for histidine tag removal following purification if desired.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 601
    Illegal BamHI site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 964


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Categories
Parameters
None